Figure 6. 1 Cell linedependent differences in transfection efficiency. Invitrogen product line such as Lipofectamine 2000 reagent and Lipofectamine 3000 reagent were used to transfect 17 cell lines with a GFPexpressing plasmid in a 24well plate format, using 0.
5 g plasmidwell and the recommended protocols for each reagent. Day 2: Remove transfection media from dish. Add another 10 ml fresh media (slowly pipet media along edge; cells will dislodge by addition of media by pipet).
Grow for 3 Effectene Transfection Reagent is an innovative nonliposomal lipid formulation that is used in conjunction with a special DNAcondensing enhancer and optimized buffer to achieve high transfection efficiencies.
transfection efficiency with S2 cells by using Effectene Transfection Reagent, a nonliposomallipid reagent from QIAGEN. The ability to efficiently and reproducibly UMMS RNAi Core Facility Protocol: Production of TRC or pGIPZ Viral Supernatant We routinely use QIAGEN Effectene Transfection Reagent, which works very well for us. Detailed protocols are provided with the kit. Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types.
Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. Effectene Transfection Reagent. For DNA transfection of primary cells and sensitive cell lines. Read More. HiPerFect Transfection Reagent.
For transfection of eukaryotic cells with siRNA and miRNA. Read More. PolyFect Transfection Reagent. For fast and easy DNA transfection of standard cell lines.
Sigmas Universal Transfection Reagent is a unique formulation of a biodegradable, proprietary cationic polymer conjugated to a cationic lipid. Universal Transfection Reagent is used for transfection of nucleic acids into common eukaryotic cell lines. Effectene Reagent is used in conjunction with the enhancer and the DNA condensation buffer (Buffer EC) to achieve high transfection efficiency.
In the first step of EffecteneDNA complex formation, the DNA is condensed by interaction with the enhancer in a definedbuffer system. Effectene Reagent is then added to the To set up for the stable cell line selection, split cells and transfect with your construct.
The following day, replace the standard media with media containing G418. Transfection of HeLa cells is carried out using the Effectene transfection reagent (Quiagen, Valencia, CA). DNA (2 g) is placed in a sterile 1. 5ml microfuge tube.
DNA (2 g) is placed in a sterile 1. 5ml microfuge tube. Reagents. the next generation in lipid technology Effectene Transfection Reagent (1 ml) Effectene Transfection Reagent (4 x 1 ml) For 40 transfections in 60 mm dishes or 160 transfections in 12well plates For 160 transfections in 60 mm dishes or 640 transfections in 12well plates PolyFect Transfection Reagent.
5 QIAfilter Mega Polyfectine is a latest developed nanoparticalebased transfection reagent by Biowit Technologies. It exhibit s superior transfection efficiency and significantly lower toxicity d uring transfection process. Effectene Transfection Reagent. For DNA transfection of primary cells and sensitive cell lines.
Kit contents: 1 ml Effectene Reagent, Enhancer, Buffer. for 40 transfections in 60 mm dishes or 160 transfections in 12well plates. Benefits: High efficiency in the presence of serum. Efficient transfection with low DNA amounts. Effectene Transfection Reagent (Qiagen). Transfection was performed as described by the manufacturer.
Briefly, the cells were split the day before transfection to maintain the viability. On the day of transfection, the cells were harvested by centrifugation, the supernatant was removed and